Human Retrovirus Protocols: Virology and Molecular Biology (Methods in Molecular Biology) (Methods... by Tuofu Zhu Sales Rank: 1681524 List Price: $200.00 $200.00 At Amazon on 10-16-2008.

Hardcover: 512 pages Publisher: Humana Press; 1 edition May 17, 2005 Language: English ISBN-10: 1588294951 ISBN-13: 978-1588294951 Product Dimensions: 9.1 x 6.1 x 1.3 inches Shipping Weight: 2 pounds Product Review "More than 20 years after the discoveries of HTLV and HIV, there appears to be a need for a consolidated reference of many of the standard and not-so-standard protocols used to characterize the human retroviruses. This book, containing some 37 detailed protocols provided by multinational authors in two parts, does an admirable job. Part I, therefore, includes guidance on isolation of HTLV, HIV and Human Foamy Virus (HFV) from peripheral blood, lymph nodes, CNS tissue and genital secretions (semen, vaginal fluids). It also describes a variety of molecular methods (largely PCR-based) to assess viral levels, integration status and drug sensitivity, plus quantification by antigen detection. At some 313 pages, Part I forms the substantial portion of the book. Part II (168 pages) guides on assessing cell and receptor tropism, construction and utilization of molecular clones and also use of microarrays to look at host and viral gene expression. The book on the whole is well illustrated with clear photographs and diagrams to support the detailed methodology, and the references at the end of each protocol are comprehensive. "-SGM Quarterly Product Description A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection. Human Retrovirus Protocols: Virology and Molecular Biology (Methods in Molecular Biology) (Methods... Available from Amazon Price: $200.00 Updated on 10-16-2008.

Search: All Products Books Magazines Popular Music Classical Music Video DVD Toys & Games Baby Computer & Video Games Electronics Software Tools & Hardware Outdoor Living Kitchen & Housewares Camera & Photo Wireless Phones Keywords:

NOTICE: All product prices, availability, and specifications
are subject to verification by their respective retailers.

Copyright © 2008,Dominant Systems Corporation
info@medvolumes.com         Privacy Policy
Last Modified : 10-16-2008